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GSH depletion causes ferroptosis in RPE cells To evaluate the mechanism of cell death in GSH-depleted cells, we treated RPE cells with various cell death pathway inhibitors including ferroptosis inhibitors ferrostatin-1 (Fer-1, 8 M), liproxstatin-1 (Lip-1, 600 nM), iron chelator deferoxamine (DFO, 80 M), pan-caspase inhibitor z-VAD-fmk (30 M) to inhibit apoptosis, autophagy inhibitor 3-methyladenine (3-MA, 10 mM), and lysosomal inhibitor bafilomycin A1 (Baf-A1, 75 nM)

These data support the calculation of the Maximum Recommended Starting Dose (MRSD) the starting dose for the first administration in humans, derived from preclinical data and converted to a human equivalent dose using internationally agreed methods

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Nakano, M., Murayama, Y., Hu, L., Ikemoto, K., Uetake, T., & Sakatani, K

Comparative analysis of glutamine and liposomal glutathione supplementation on immune function