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Then, the washed cells, the number of which was consistent with that in 20 L of the overnight culture, was added to 5 mL of the chemically defined medium in a threaded test tube, followed by the static incubation at 37 C
If the reconstituted solution shows any discoloration, particles or if anything similar is observed, discard it immediately
It puts vitamin B12 straight into your blood, skipping the usual digestion steps
*Correspondence: Gang Liu [email protected] These authors have contributed equally to this work This article was submitted to Animal Reproduction - Theriogenology, a section of the journal Frontiers in Veterinary Science ORCID: Shubin Li orcid.org/0000-0002-6865-9667 Jingyu Ren orcid.org/0000-0002-0048-3052 Wenqi Zhang orcid.org/0000-0003-4891-500X Yanfeng Dai orcid.org/0000-0001-7147-1748 Gang Liu orcid.org/0000-0002-5180-3765 Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
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