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It is intended for analytical, receptor-binding, and biochemical research conducted by qualified professionals in controlled laboratory environments
Proteins were clarified by centrifugation, quantified by BCA assay, reduced with DTT, alkylated with iodoacetamide, diluted and sequentially digested with LysC (1:50, 2 h) and trypsin (1:50, 14 h)
doi:10.1111/j.1365-2036.2008.03788.x 55Wright S
Unfer, V., Facchinetti, F., Orr, B., Giordani, B., & Nestler, J
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