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First, GABA is initially excitatory

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About 1 g of total RNA was converted to cDNA using random primers and Maxima H minus reverse transcriptase (both from Thermo Fisher Scientific)

In agreement with observed resistance in KO mice, levels of phosphorylated AMPK subunit (at Thr172 residue) were higher in the livers of KO mice than in WT mice in all diet groups (Fig