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Primary antibodies (Table S1) diluted in TN buffer (0.1 M TrisHCl, 0.15 M NaCl, pH 7.5) were reacted overnight at 4 C

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Cleavage of full-length proteins in cell lysates HEK293T cells were plated in 12-well plates at 1 10 5 cells per well in 1 ml

Weight regain following GLP-1RA discontinuation is common and often rapid, indicating that pharmacological appetite suppression does not permanently reset biological weight-defense systems ( Postbiotics introduce a contrasting paradigm

It is approved for treating hyperglycemia in patients with T2DM in many countries (105, 106)