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However, the test membranes are also heavily stained orange by the 5-Amino-1MQ solution, demonstrating substantial interference from the sample itself

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Then, in 2015, Lee and colleagues identified a previously overlooked open reading frame within the 12S ribosomal RNA gene of mtDNA and found that it encodes a functional peptide, MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA-c) [1]

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3 The best answer for most people is to use both together through the week