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Both hexyl moieties are oriented parallel and fill the H-subsite of the enzyme's active site
Topical vitamin C in concentrations between 10-20% is generally safe for continuous use, while the sustainability and safety of prolonged glutathione use for cosmetic purposes requires more research
*Correspondence: Smita Kumar, [email protected] This article was submitted to Plant Traffic and Transport, a section of the journal Frontiers in Plant Science Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
METHOD DETAILS Sample preparation FOXO4 FHD (95-195) was subcloned into the pET His6 Tobacco Etch Virus (TEV) LIC cloning vector (2B-T, obtained from Scott Gradia, Addgene Plasmid #29666) and transformed into BL21 (DE3) cells
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