Known as the sunshine vitamin, Many people are deficient due to limited sun exposure, dietary gaps, or lifestyle factors, which can lead to fatigue, low immunity, and weakened bones
It aids in reducing oxidative stress, which, when levels are too high, can lead to multiple diseases, such as diabetes, cancer and rheumatoid arthritis
Surviving Sepsis Campaign: International guidelines for management of sepsis and septic shock 2021
Over time, this can contribute to visible changes in skin appearance, including uneven pigmentation
The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat