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For lower doses (100-200 mcg), 2.5 mL works well, giving you a 2 mg/mL concentration where 100 mcg is a clean 5 units

Signaling supports local balance in tissues that absorb load, especially tendons, ligaments, and the gut

It probably entails the need to monitor a protein intake (e.g., high vs low) albeit in a calorie deficit

For IM, stretch it taut

Lower activity of oxidative key enzymes and smaller fiber areas in skeletal muscle of post-obese women Am J Physiol: Endocrinol Metab 1998 275 : E487E494