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Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice

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Statistical significance was determined by one-way repeated measures analysis of variance

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IMPACT OF HEAVY METAL TOXICITY IN PLANTS Several metals/metalloids such as Cd, Pb, Cr, As, Hg, Ni, Cu, and Zn cause toxicity in plants [15]