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Antibody-bound tracer was separated from the unbound tracer by precipitation in solution provided in the kit containing 3% PEG and 0.05% Triton X-100 in 0.05M Phosphosaline with 0.025M EDTA and 0.08% sodium azide

The detailed procedures for intestinal content metagenomic DNA extraction, sequencing, and the bioinformatic pipeline (including taxonomic and functional composition analysis) were performed according to our published article [37]