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For immunofluorescence, sections (5-m thick) were incubated with 5% horse serum for 30 min to block unspecific antibody binding, then incubated overnight at 4 C in a mixture of anti-AR (Santa Cruz: sc-7305) and anti-Prrx1 (Santa Cruz: sc-293386) primary antibodies, diluted 1:100 in 1% horse serum/TBST (Tris-buffered saline containing 0.1% Tween-20)
The questionnaire demonstrated high internal consistency (Cronbach's alpha = 0.89) and excellent testretest reliability (ICC = 0.97
It has been theorized that IGF-1 LR3 might promote cellular proliferation and differentiation, making it a valuable tool for studying tissue development and repair
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