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Science 278 , 873876 (1997)

The second portion of the left kidney was homogenized in RIPA buffer and utilized for western blot analysis of kidney tissue TNF- protein expression

Work through these troubleshooting steps in order, making one change at a time and allowing two weeks to assess each modification's impact

Alongside VEGF, HIF-1 also induces other angiogenic mediators such as angiopoietins, platelet-derived growth factor (PDGF), and stromal-derived factor-1 (SDF-1), which collectively orchestrate the complex remodeling of the tumor vasculature (166, 169)