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A rapid sample preparation procedure was developed for efficient extraction of ipamorelin from dried blood spots Data Dependent Acquisition (IDA) generated reproducible and comprehensive quality TOF MS and TOF MS/MS spectra of the most abundant precursor/candidate ions, enabling reproducible peptide fragmentation Data generated using IDA method allowed reliable characterization and quantitation of ipamorelins precursor ion and peptide fragments Workflow showed excellent sensitivity, with an LOD of 2.5 ng/mL and LOQ of 5 ng/mL for ipamorelin extracted from DBS while maintaining accuracy and precision Assay showed excellent linearity with an R 2 value of 0.99499 for ipamorelin across the calibrator series ranging from 5 to 100 ng/mL Developed workflow can be modified to include larger panel of analytes, including additional GHRPs listed on the World Anti-Doping Agency (WADA) Prohibited List

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