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Cooling to 0 , stirring for 30min, adding 0.22g of HOSu, stirring and reacting for 12 hours at the temperature, filtering, collecting filtrate, and spin-drying the filtrate to obtain a crude Boc-His (Trt) -Aib-OSu product

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Once in the bloodstream, the cells absorb it

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Thiobarbituric acid reactive substances (TBARS) are biomarkers of oxidative stress, the quantification of which reflects the MDA content