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Reverse-phase HPLC purity assessment (typically by area-percent at 210220 nm) and mass-spectrometric confirmation of molecular weight against the appropriate species (free tripeptide or copper complex) are standard practice

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Treasure Island (FL): StatPearls Publishing

Following 8 h of magnetic stirring at 25 C, mPEG5000-OH (1.35 g, 0.27 mmol) was incorporated into the reaction system