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All qRTPCR assays were performed on the QuantStudio 5 Real-Time PCR system (Applied Biosystems, CA, USA) with Power-Up SYBR Master Mix (Applied Biosystems, CA, USA)
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Total glutathione values (BSO did not cause oxidation of glutathione, data not shown)
Many of the animal studies note that BPC-157 appears to be generally safe
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