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For cell sorting of Lenti-X 293T, cells were rinsed twice with 500 L PBS and then treated with 300 L Non-enzymatic Cell Dissociation Solution (13151014, Gibco) for 46 min at 37C to detach cells

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for instance, certain vitamin B-dependent epilepsies necessitate doses up to 100-600 mg per day to control seizures, with long-term supplementation of about 30 mg daily for life to prevent symptom recurrence

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