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Analysis of single-cell RNA sequencing Single-cell RNA sequencing (scRNA-seq) data (HRA000425) were downloaded from Genome Sequence Archive (SRA)-Human databases in China National Center for Bioinformation 16 , converted into FASTQ format using fasterq-dump software (sratoolkit 3.0.2, NCBI, NIH, Bethesda, MD, USA), converted into a matrix data file using Cell Ranger software version 3.0.2 (10xGenomics, Pleasanton, CA, USA), converted into Seurat objects using Seurat 5.0.1 software (Satija lab, Lower Manhattan, New York, NY, USA), and plotted for t -distributed stochastic neighbor embedding ( t -SNE) visualization

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Using high-resolution electron spray ionization mass spectrometry (HR-ESI-MS), the presence of GSSG ([M-H] = 611.1442) was verified (Supplementary Fig

If you are using a glutathione supplement, check the label for specific instructions, as some forms (like liposomal glutathione) are liquid and others are capsules