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The addition of glutathione precursors and synergistic antioxidants creates a multi-faceted approach to cellular protection and detoxification
At 0, 3, 7, and 10 days, the tissues were fixed, and cryosections were used for in situ apoptotic staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), according to the manufacturers instructions (Servicebio, Beijing, China)
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Following the latter protocol, we attained vacuum distillation of EBN, but failed to obtain a nitrone fraction that was free of trace amounts of nitroxides, which ultimately interfere with EPR spin-trapping experiments
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