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The adduct was purified by size-exclusion chromatography and concentrated to ~10 mg ml 1
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In addition, 2-DG uptake was markedly suppressed in tumors derived from U87 cells with the knockout of endogenous DHHC9 or GLUT1, and these suppression effects were abrogated by reconstituted expression of WT rDHHC9 or rGLUT1, but not rDHHC9 C169S or rGLUT1 C207S (Supplementary Fig
The effects of our shots vary between individuals, but are typically felt for about one week
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