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Cyclosporine in acute myocardial infarction
The occurrence of ferroptosis in mature erythrocytes has not been confirmed and it seems quite challenging, since ferroptosis studies suffer from the lack of well-established markers and evaluation of this cell death requires a complex approach with identification of typical cellular and subcellular morphological alterations, genetic hallmarks, biochemical changes, expression of certain ferroptosis-related proteins, etc
Because GPx contains a selenocysteine residue in its active site, selenium, which is required in trace amounts in the diet, is often described as an antioxidant nutrient (see Chapter 11 )
Next, sections were blocked in 3% normal donkey serum for 1 h, followed by incubation in anti-pSTAT5 Tyr694 primary antibody (1:1000