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We could express a protease of interest under an inducible promoter and select in the presence of chloramphenicol in Escherichia coli expressing a large library of peptide substrates present in the linker between T7 RNAP and its fused T7 lysozyme inhibitor
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Cyclic AMP: master regulator of innate immune cell function
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Can Glutathione Help with Acne Scars and Hyperpigmentation
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Transformed yeast cells were screened for high-expression clones by small-scale expression experiments (5 ml cultures), with levels for each clone determined by immunoblotting
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