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For flow cytometry, cells from the third villus fraction and crypts were fixed with 4% paraformaldehyde for 15 minutes at room temperature, washed twice with PBS, and permeabilized with saponin-based buffer
10.2337/db16-0058 65 HayashiN.NakamoriS.HiraokaN.TsujieM.XundiX.TakanoT.et al (2004)
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