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Indeed, inflammaging is associated with cell ROS over-production leading to oxidation/damage of cellular components, enhanced inflammation, and activation of cell death pathways
PMC 6673945
More recent work linked long-term NS work ( 10 years) to significant hypomethylation in 85 CpG sites, including loci in ZFHX3, a gene involved in circadian rhythm regulation and tumor suppression, and ATXN7, associated with transcriptional control and cancer susceptibility 231
After sonicating (1400 rpm, 20 C, 5 min), the extracts were cleared by centrifugation (17,000 g , 20 C, 10 min), and the lipids in the supernatant were analyzed by LC-MS
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