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Furthermore, their AMs have lower expression levels of proliferator-activated receptor (PPAR)-, an important player in combatting oxidative stress during acute lung injury, higher expression levels of expression of NADPH oxidases, which further promote oxidative stress and inhibit phagocytosis, and higher levels of TGF-, a big driver of tissue fibrosis (113116)
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Then, membranes were incubated with the appropriate primary antibodies at 4C overnight
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As evidenced in db/db and ob/ob mouse models (Thaiss et al., 2018), sustained hyperglycemia activates intestinal epithelial glucose transporter 2 (GLUT2) and downregulates the expression of zonula occludens-1 (ZO-1), increasing intestinal permeability
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