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The analytes were separated on chromatographic column by isocratic elution (98% phase B) at the flow rate of 0.3 mL/min
In line with the covalent binding mechanism, in vitro and cell-based thermal-shift assays showed that C1-27 induced a negative shift in the melting temperature indicating protein destabilization on binding (Supplementary Figs 11 and 15)
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