Therefore, formulations presenting hemolysis values below 5% were considered acceptable for further biological evaluation
The cells were resuspended in relaxation buffer (100 mM KCl, 3 mM NaCl, 3.5 mM MgCl 2 , 1 mM ATP and 10 mM PIPES pH 7.3) containing protease inhibitors (cOmplete EDTA-free, Roche)
Vitamin C is an antioxidant which scavenges free radicals in the body and protects tissues from oxidative stress [10-12,16-20]
Neurons can then refill their TCA cycle from glutamine via metabolism to glutamate and then -ketoglutarate catalyzed by glutaminase and glutamate dehydrogenase, respectively
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