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Coverslips were washed with TBST 3 times then incubated with DAPI and donkey anti-rabbit Alexa Flour 488 (Invitrogen #a21206) for 1 hour

To realize this vision, future research maybe focus on standardizing the measurement of eryptosis in clinical settings, validating its role as a predictive biomarker, and conducting well-designed clinical trials to evaluate the efficacy and safety of novel anti-eryptotic agents

Supplemental lipoic acid also decreased the serum concentrations of some (IFN-, ICAM-1, TGF-, IL-4), but not all markers (TNF-, IL-6, MMP-9), cytokines and other inflammation (98)