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The annealed PTEN-shRNA or GPX4-shRNA oligonucleotides were cloned into linearized lentiviral vector pLVshRNA-EGFP (2 A) Puro using BamHI and EcoRI digestion and T4 DNA ligase ligation according to the manufacturers protocol
Severe kidney disease
Pi LQ, Jin XH, Hwang ST, Lee WS
Histological examinations of the liver also revealed that tamoxifen treatment induced pathological changes in the liver and increased the tumor necrosis factor levels
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