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We combined the single-cell transcriptome data obtained from all 3 donors and performed unsupervised clustering on the combined data set using Seurat (36) to create various cell clusters and then used respective hormone markers to identify each cluster (Supplemental Figure 3, B and C)

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Next, the wells were washed with wash buffer, followed by addition of 100 L of Detection Reagent B working solution to each well and subsequent incubation for 1 h at 37 C after covering with plate sealer

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