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The tissues were analyzed on the Ventana Discovery Ultra automation system (Ventana Medical Systems) for GLP-1 receptor expression using rabbit-anti-mGLP1R (Abcam, ab218532, lot: GR3231665-2) at a concentration of 2.7 g ml 1

Whether this dose-response relationship extends to addiction outcomes remains to be determined, but the theoretical rationale is compelling

For the in vitro ubiquitination assay, 0.01 g E1, 0.03 g E2, 0.03 g E3, 0.1 g Pelo, and 0.1 g ubiquitin (ThermoFisher Scientific) were incubated in ubiquitination buffer (1 M Tris, pH 7.2, 100 mM ATP, 200 mM MgCl 2 , 40 mM DTT, 20 ) at 30 C

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