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The incubation period for each tube was 20 min at 25C, and the reaction was halted by adding 0.5 mL of 0.8 mM CuCl 2 solution to each tube every 30 seconds
Slowly depress the plunger to deliver the medication at a steady pace about 1 ml every 10 seconds is a good guideline for minimizing discomfort
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11 Conversely, in a study assessing a cream containing 0.1% GSH-C4, 0.5% beta-glycyrrhetic acid, and 10% azelaic acid, 10 percent of the participants discontinued due to lower skin irritation
