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Real-time fluorescence quantification Total RNA was extracted from kidney and intestinal tissues, as well as HK-2 and HIEC-6 cells, using the Trizol method (Invitrogen, USA)

46 Clinical manifestations Identification of APAP overdose is critical, as significant morbidity and mortality may be prevented with early therapy

Conflict of interest JPR received research support for this study and travel funds to present previous research findings from Kyowa Hakko Bio Co., Ltd