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After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h
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began to investigate a multi-state outbreak of fungal meningitis and other infections among patients who had received contaminated steroid injections from New England Compounding Center (NECC) of Framingham, Massachusetts
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