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Protein expression and purification SLC33A1 and SLC33A1 MAP Genes encoding human SLC33A1 and SLC33A1 MAP were subcloned into a pBacMam expression vector with C-terminal eGFP tag fused through a short linker containing a PreScission protease site 58

Acute studies generally show that activating ARC AgRP/NPY neurons increases food intake, while activating ARC POMC neurons suppresses food intake with the opposite profile seen with their respective inhibition [134,135,136,137,138]

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By monitoring the absorbance readings over these time intervals, the study aimed to assess any potential changes in the stability of Ellmans reagent product