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R., & Hosseini, S
10.1016/j.scitotenv.2024.176476 94 LimC
Melanocortin activity in the amygdala influences alcohol intake
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Electron microscopy sample preparation and data acquisition A 3 l volume of 5 mg ml 1 purified SLC33A1 MAP was mixed with Pmab1 Fv-clasp at a 1:1.5 molar ratio and 20mM GSSG, incubated for 30 min on ice and then applied to glow-discharged Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil) or Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 grids before being plunged into liquid-nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (FEI Thermo Fisher)
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