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CD4+ T-cell counts were determined by flow cytometry using the Trucount Kit in FACSCanto II instruments (BD Biosciences, San Jose, CA, USA)

Coelentrazine-h (Yeasen Biotech) was added to a final concentration of 5 M for 5 min before bioluminescence resonance energy transfer (BRET) readings were made using an EnVision plate reader (PerkinElmer)

[4] Understanding this pharmacological basis helps patients recognise that bloating, whilst uncomfortable, is a predictable consequence of how these medications work rather than an indication of treatment failure

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What monitoring do I need if I take both