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Flow cytometry Isolated lamina propria cells were stained with the following fluorescence conjugated anti-mouse antibodies in FACS buffer: Lineage Cocktail-APC (BD Biosciences, Bergen County, NJ, USA, #558074, 1:200)), CD45-FITC (BioLegend, #103108, 1:200), CD4-PerCP/Cy5.5 (BioLegend, #116012, 1:200), CD90.2-BV510 (BioLegend, #140319, 1:200), IL-7R-PE-Cy7 (BD Biosciences, #560733, 1:200), RORt-BV421 (BD Biosciences, #562894, 1:200), and Live/Dead (Invitrogen)

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While the appeal for this latest trend in medical weight loss is understandable, its not standard medical protocol

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