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A14527, Gibco) were cultured in Expi293F Expression Medium (Gibco) in baffled flasks on an orbital shaker platform (Thermo Fisher Scientific) set to 125 RPM at 37 C and 8% CO 2
The solution should appear uniformly blue-tinted and completely clear
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In addition, knockdown of xCT exacerbates Ang II-induced cardiac hypertrophy, fibrosis, and dysfunction in mice