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The researchers applied water soluble black ink to the plantar surfaces of the rats hindpaws prior to walking down the walkway from its entrance and into the shelter

The membranes were blocked using Bullet Blocking One for Western Blotting (Nacalai Tesque), incubated overnight at 4C with the primary antibody, and then incubated with an HRP-conjugated secondary antibody

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This is not a small problem when you are working with a vial that contains enough peptide for four to twenty-four weeks of research, depending on your dose

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