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Nanospray voltage was set at 2.10 kV with the heated capillary temperature at 275 C
The multiple conformations observed from the single-particle EM analysis of the purified Fab7F38semaglutideGLP-1RGsNb35 complex most likely represent different agonist-bound ECD conformations, however some 2D classes are also compatible with the closed conformation observed in our inactive crystal structure (Supplementary Fig
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