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People also lost weight during the course of the study

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After 6 h of treatment, cells were washed 3 times with warm PBS and scraped in lysis buffer (2 % SDS, 50 mM Tris/HCl pH 8.5, 10 mM TCEP, 40 mM CAA, 1 mM PMSF, 1x cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail), followed by boiling at 95 C for 10 min, sonication for 5 min and again boiling for 5 min at 95 C