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The membranes were washed three more times, developed with Ultra Signal ultra-sensitive ECL chemiluminescent substrate (Beijing 4A Biotechnology Co., Ltd.), and imaged with the Vision Works system (Analytik Jena AG, Germany)
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According to the US National Heart, Lung and Blood Institute, a sign of vitamin B12 deficiency is diarrhoea If a person lacks B12 their red blood cell count will be lower and their nerves risk becoming damaged
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