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Mistake 2: stopping the protocol too early Disc tissue heals slowly, much slower than muscle or even bone

Research Background In preclinical research settings, DSIP has been examined across laboratory models, often in the context of: Neuropeptide and hypothalamic signalling studies Sleep-wake cycle and circadian rhythm research models Stress-response and neuroendocrine pathway investigations Published research summaries and references can be accessed via public scientific databases such as PubMed

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After 1 h, the medium was removed and cells were washed with 1 PBS twice before trypsinization

The catch is that both slow digestion, so GI side effects can stack