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Chromatographic separation was conducted on a Brownlee SPP C18 column (2.1 mm 75 mm, 2.7 m particle size, Perkin Elmer, Waltham, MA) using mobile phase A (100% H2O containing 0.1% formic acid and 1% of 1 M NH 4 OAc) and B (1:1 acetonitrile:isopropanol containing 0.1% formic acid and 1% of 1 M NH 4 OAc)

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doi: 10.1080/15548627.2023.2170960, 126 XiaoY.YuanY.YangY.LiuB.DingZ.LuoJ.et al

Unlike standard protocols that may show effects within days, micro-dosing often requires weeks before meaningful changes become apparent