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37 In terms of antimicrobial activity, berberine demonstrated strong effects against dysbiotic bacteria in IBS models, reducing the abundance of Escherichia/Shigella and Proteobacteria while enriching beneficial taxa such as Lactobacillus and Ruminococcus

Given the complexity of the analysis, an LCMS/MS analysis was subsequently performed after the HPLC analysis to ensure accuracy

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The free peptide chelates a Cu ion with high affinity, generating a deep cobalt-blue complex that is the workhorse molecule of modern copper-peptide research

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