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Side-effect profile is similar to GLP-1 agonists
After blocking, sections were incubated overnight at 4 C with anti-GLP-1 (1:200), followed by incubation with Alexa Fluor 594-conjugated secondary antibody (1:500) under light protection
While the 200-ns replicates served as control runs to verify result reliability, the final 80-ns segment of the repetitive MD simulations were used for binding free energy calculations
J Invest Dermatol 136:19551960 Guitera P, Pellacani G, Crotty KA, Scolyer RA, Li LX, Bassoli S, Vinceti M, Rabinovitz H, Longo C, Menzies SW (2010) The impact of in vivo reflectance confocal microscopy on the diagnostic accuracy of lentigo maligna and equivocal pigmented and nonpigmented macules of the face
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