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NNT activity contributes to 45% of the total NADPH in mitochondrial pool, indicating a significant role of NNT for NADPH pool maintenance, 111 and NADPH obtained by NNT is also used for the reductive carboxylation of -KG to isocitrate mediated by IDH2
PMID 2600821
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Finally, in the third aliquot, protein precipitation prior to treatment of the supernatant with TCEP and IAM, followed by analysis of CAM-S-CAM-TPP, quantified total L MW SS /L MW S(S) n S present
Concentration, delivery method, duration, and the specific outcome being measured all shift the GHK-Cu timeline
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