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Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution
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Furthermore, it has been reported that lysosomes, peroxisomes, Golgi apparatus, endoplasmic reticulum (ER), and ribosomes are also cleared from the erythroid lineage cells in an autophagy-dependent fashion [32, 36]
